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R.BetheaJ

Environ Sci Technol 49:59565964

In wild type IDH cells, (D)-2HG is produced from hydroxyacidoxoacid transhydrogenase that converts -hydroxybutyrate to succinic semialdehyde while reducing 2KG to (D)-2HG [83]

For example, after synthesis and cleavage to give the peptide acid, the free acid is converted to an activated ester by an appropriate carboxyl group activator such as dicyclohexylcarbodiimide (DCC) in solution, for example, in methylene chloride (CH2Cl2), dimethyl formamide (DMF) mixtures