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Measurement of ALDH2 enzymatic activity in mouse brain The mice were administered ALA (20 mg/kg) concomitantly with 4-MP (10 mg/kg), and 90 min later, the brain total fractions of the mitochondrial and cytoplasmic proteins were separated as described previously (Bunik et al., + , 50 mM FA and 20 l of the brain mitochondrial fraction

In line with these findings, in rats the activity of ADH was significantly reduced in diabetic models as well as in animals fed by high-carbohydrate, fat-free diet 100

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(ii) the link between oxidative stress, GPxs, and the metabolic syndrome