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Methods Protein expression and purification The codon-optimized gene encoding full-length human ABCC2 (Uniprot: Q92887) was synthesized (Sangon Biotech, Shanghai) and cloned into a modified pCAG vector harboring a C-terminal Flag tag (DYKDDDDK) and an 8 His tag using a ClonExpress II One Step Cloning Kit (C113-02, Vazyme Biotech co., Ltd)

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