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Dienemann S, Schmidt V, Fleischhammer T, Mueller JH, Lavrentieva A (2023) Comparative analysis of hypoxic response of human microvascular and umbilical vein endothelial cells in 2D and 3D cell culture systems

Pellets were grinded with pestles in 100 l of the same buffer, cells were lysed by two freeze-thaw cycles and insoluble material was removed by filtration in a COSTAR R Spin-X centrifuge tube filter