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Fatty acids in foods and their health implication

10.3389/fimmu.2022.820718 43 HuoY.YueD

TNF and PGs either interact with certain processes in the stimulussecretion coupling machinery within astrocytes (Domercq et al., 2006

The medium was removed and cells were washed with 1 PBS twice before trypsinization

This might be due to unfavourable ionisation properties of the disulfide-containing peptide or the disulfide might negatively affect tryptic cleavage of the respective peptide before mass spectrometric analysis