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For future clinical applications, large-scale and long-term studies are necessary to establish more consistent clinical outcomes and validate the actual efficacy of hydrogen therapy

2.6 Metabolomic sample collection and analysis Oocyte sample collection and metabolomic analysis were performed based on methods from Zhao et al

Figure 7 Cell Apoptosis and DNA Fragmentation At 13 weeks, there were no significant changes in capase-3 and TUNEL positive cell numbers among the three female experimental groups, although cortex capase-3 and TUNEL positive cells in the SE offspring were more than doubled than the SHAM offspring (Figures 8D,H)

85 LeeJ.-M.LeeJ.SonB.-S.JangM.-U.LeeS.-W.HurhB.-S.et al (2017)