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RNA extraction and qRT-PCR The purified total RNA was extracted by Trizol reagent, and then reverse-transcribed by using the Fastking RT Kit (Tiangen, Beijing, China)

J Leukoc Biol 116:13241333 Chu VT, Beller A, Rausch S, Strandmark J, Zanker M, Arbach O, Kruglov A, Berek C (2014) Eosinophils promote generation and maintenance of immunoglobulin-A-expressing plasma cells and contribute to gut immune homeostasis

Moreover, insulin facilitates the conversion of liver glucose into glycogen for storage, reducing blood glucose levels (Villagarca et al., 2018)

First, proper storage: lyophilised powders should be kept sealed in a cool, dry place away from light and moisture, while reconstituted solutions require refrigeration after opening, as compound potency degrades quickly without it

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