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10.1016/j.bbr.2006.11.044 Behav

Decreased arginine levels or excessive NO production suppress T cell proliferation and promote Treg differentiation

Katoh et al

Diabetologia 58 , 3136 (2015)

Cell proliferation assay The viability of GC-1 spg, TM3 Leydig, and TM4 Sertoli cells was measured using an MTT assay EZ-Cytox kit (Daeil Lab Services Co., Seoul, Korea) according to the manufacturers instructions Each cell was seeded in 24-well plates at a density of 0.5 10 5 cells/well and incubated at 37 C for 12 h

3.2 Protein oxidation products 3.2.1 Advanced oxidation protein products Advanced oxidation protein products (AOPPs) constitute a class of complex protein compounds composed of dimethyltyrosine, pentosidine, and carbonyl residues