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SDS_PAGE gel was blotted and stained directly with an HRP-linked anti HIS-ab (lower right panel), or indirectly using mouse M2 anti-Flag antibody followed by anti-mouse HRP-conjugate (loer right panel)

By contrast, in a resistant cultivar the GSH pool homeostasis was maintained throughout the bacterial attack

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Incubation with 13 C 3 -serine as an extracellular serine source for 6 h resulted in almost complete labeling of the intracellular serine pool (Fig

This reduction in GSH levels is often correlated with increased markers of oxidative damage, such as malondialdehyde and total carbonyls, indicating a higher degree of lipid peroxidation and protein oxidation (82)

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