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The top hits include Trp-catabolism the OVX animals plasma showed high level of KYN and that was reduced by EB-treatment, the KYN/Trp ratio was significantly high in the OVX animal compared to the OVX+E2 treated group

The optical density value of a sample was determined at a wavelength of 450 nm using an Infinite M200 PRO plate reader (Tecan, Austria)

Where indicated, the protein synthesis inhibitor cycloheximide (CHX) (Sigma-Aldrich) and the proteasome inhibitor MG132 (Sigma-Aldrich) were added to HEK293 cells cultured with a final concentration of 25 g/ml and 10 M respectively in a time-course experiment

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Fibrosis-associated signaling molecules are differentially expressed in palmar connective tissues of patients with carpal tunnel syndrome and Dupuytrens disease