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Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen 0.5 mM L-glutamine, and 5mM cytosme-D-arabinofuranoside from Sigma added at 2 days in vitro

Distribution and specific binding of 3 H-DSIP

Disinfect the rubber stopper with alcohol and allow complete evaporation before drawing bacteriostatic water

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This cycling approach allows the body to benefit from the peptide repair signaling during the active phase and then consolidate those gains during the off phase using its own endogenous repair mechanisms