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Draw up 6 ccs of bacteriostatic water in syringe - making 7 ccs total in syringe 5

Protein extracts were then subjected to SDSPAGE and transferred to Immobilin-FL polyvinylidene fluoride transfer membrane

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Immunoprecipitation Since no antibody for phospho-CAR is available, we immunoprecipitated CAR from total liver lysates and used a general anti-phospo antibody for Western blot analysis

The following table provides a comparative overview for researchers considering these two compounds for their in-vitro studies

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