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The young leaves are particularly important in pharmacological research, as their components are more potent than those found in mature leaves (218, 219)

bassiana begins with adhesion to the host pest, penetration of cuticle, and colonization of the pest heaemocoel (Wojda et al., 2009)

IHC staining of human kidney using 55463-1-AP Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 10x lens)

10.1002/jat.727 7 GuptaA.AtanasovA

Selecting the appropriate detection method, protein expression system and conditions for sample preparation are vital

Acknowledgements This work was supported by the National Natural Science Foundation of China (grant no 81500354), Beijing Municipal Natural Science Foundation (grant no Z13110200400000) and Wu Jieping Medical Foundation (grant no 320.6750.14331)