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Description

Histology FFPE tissue microarray (TMA) construction Colon biopsies from patients were fixed in 10% neutral-buffered formalin (Millipore Sigma) for 1624 h, washed in PBS three times, then placed in 70% ethanol and stored at RT until paraffin-embedding by the Biospecimen Resource Program (BIOS) at UCSF

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Many protocols use 4 to 6 acquisition days with several trials per day, then a probe trial after criterion or the final acquisition day

Given that multiple factors can influence the composition and proportion of the GM, and given the discrepancies among different studies regarding the GM of AIH patients, more rigorous and long-term cohort studies are needed [85]

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