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Recombinant protein purification and in vitro binding assays GST, GST-mSIN, GST-AKT (K179D), GST-PTPN22, His-tagged PTPN22, MBP or MBP-mSIN, MBP-PTPN22 full-length proteins were expressed in Escherichia coli BL21 (DE3) cells, and the proteins were affinity purified using either glutathione sepharose TM 4B beads (GE Healthcare, GE17-0756-01) [for GST-tagged proteins] or dextran sepharose TM beads (GE Healthcare, GE28-9355-97) [for MBP-tagged proteins] or Nickel NTA agarose beads (Qiagen, 30210) for 2 h at 4 C

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In addition, IHC staining demonstrated that the intensity of 4-HNE, reflecting the lipid perioxidation, was higher after FA injection, while LA supplementation significantly downregulated the intensity of 4-HNE