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Nature 540 , 139143 (2016)

Resuspend pellet with 0.4 ml of 0.075 M TRIS base containing 1 mM EDTA and 1 mM DTT

For immunohistochemistry of activated-caspase 3 in glioblastoma, mice were transcardially perfused with 30ml of PBS followed by 30ml of 4% PFA

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