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39 Twenty-four hours after transfection, cells were plated in media containing 10% dialyzed FBS and DMEM in white, opaque 96-well plates (PerkinElmer, Waltham, MA) at a density of 150,000 cells/100 L and incubated at 37 C, 5% CO 2 overnight

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doi: 10.1038/nature08937 70 Henao-MejiaJ.ElinavE.JinC.HaoL.MehalW

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Generally, macrophages are divided into M1 and M2 subtypes, which have pro-inflammatory and anti-inflammatory effects, respectively