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Lee MS, Helms TL, Feng N, Gay J, Chang QE, Tian F, Wu JY, Toniatti C, Heffernan TP, Powis G, Kwong LN, Kopetz S

[ 13 C 4 ]GABA labelling of T H 17, iT reg and T H 1 cells Naive CD4 + T cells isolated from WT mice were polarized for 72 h under T H 17, iT reg or T H 1 culture conditions before being collected and re-seeded at a density of 2 10 6 cells per ml in a conditional medium (RPMI-1640) containing 0.5 mM [ 13 C 4 ]GABA, 0.1 mM Gln and the GABA A -R antagonist bicuculline (5 M)

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Another biochemical study revealed that pro residue at position 504 of DNA2 is crucial to stabilize the ISC

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