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Then IgG or indicated antibodies (2 g) were added to supernatants for 4 h at 4 C, captured by adding the protein A/G agarose (Pierce, 20421) before incubation for 4 h at 4 C and the beads were washed five times with IP buffer containing 500 mM NaCl

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Cheng, X., Peuckert, C

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Intriguingly, endoplasmic reticulum-related protein quality control can be either proteasome or lysosome-dependent, where the regulatory mechanisms still need further elucidation [81]

Incubation of these inhibitors with TGR in the absence of NADPH led to significantly attenuated enzyme inhibition compared to inhibition resulting from incubation in the presence of NADPH (Fig