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PMID: 111 DuschaAHegelmaierTDrholzKDeselCGoldRZaissMMet al

Ribeiro, C.M., Paradiso, A.M., Carew, M.A., Shears, S.B

Advertisement This protocol is an extension of the widely accepted PEN-FAST (penicillin allergy reported by patient, five years or less since reaction, anaphylaxis or angioedema, severe cutaneous adverse reaction, and treatment required for reaction) clinical decision tool that has been used to identify patients with reported penicillin allergy who are appropriate for DOC rather than immediate hypersensitivity skin testing, which recent data suggest has poor positive predictive value in low-risk patients

These mechanisms can potentially reduce secondary injuries and enhance clinical outcomes (Fernndez-Gajardo, Matamala, Carrasco, Gutirrez, Melo and Rodrigo, 2014)

Prior to the analysis, G422 cells were seeded at a density of 1 10 6 cells/well in a 12-well plate and allowed to adhere and proliferate overnight under optimal culture conditions