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Cells were lysed with lysis buffer (20 mM TrisHCl pH 8.0, 1% NP-40, 150 mM NaCl, 1 mM EDTA, and protease inhibitor cocktail) and lysates were centrifuged at 16,000 g for 20 min at 4 C

Tube formation, sprouting, and VEGF production are measured in response to GHK-Cu treatment

Anal Biochem 72:248254 Brengi Sary H, Khedr Abd Allah EM, Abouelsaad IA (2022) Effect of melatonin or cobalt on growth, yield and physiological responses of cucumber (Cucumis sativus L.) plants under salt stress

McAlpine CS, Park J, Griciuc A, et al

Neuron 112, 12651285.e10