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doi: 10.3389/fnano.2020.589541 89

Developmental competence of bovine embryos from heat-stressed ova

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36 , e22224 (2022)

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Cerebrospinal fluid was thawed at room temperature and immediately processed by mixing 150 L cerebrospinal fluid with 300 L of isopropanol, vortexing serial incubations of 5 min in an ultrasonic bath, 20 min on ice, and incubation at -20C for 20 min