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RNA Extraction, cDNA Synthesis, and Real-Time qPCR RNA was extracted using EasyPure RNA Extraction Kit (Transgen Biotech) and cDNA was synthesized using the TransScript One-Step gDNA Removal and cDNA Synthesis kit (Transgen Biotech)

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Peptides were further separated onto an EASY-Spray RSLC C18 column (75-m ID, 50-cm length

& Zupanc, M

These findings indicate that HepG2 ATP7B-KO cells exhibit an earlier and more robust copper-dependent upregulation of oxidative stress-related genes compared to HepG2 cells