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Samples were mixed 3:1 with 4x loading buffer (200 mM Tris pH 6.8, 8% SDS, 50 mM EDTA, 0.8% Bromophenol Blue, 50% glycerol) without reducing reagent to preserve 4HNE adducts, and boiled at 100 C for 5 minutes
Jan 27 2024;25(1):99
National Center for Biotechnology Information (US) Bertoglio D, Amhaoul H, Van Eetveldt A, Houbrechts R, Van De Vijver S, Ali I, Dedeurwaerdere S (2017) Kainic acid-induced post-status epilepticus models of temporal lobe epilepsy with diverging seizure phenotype and neuropathology
