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Concentration = 10mg 2mL = 5mg/mL If a protocol step calls for 2mg, the injection volume is: 2mg 5mg/mL = 0.4mL On a standard U-100 insulin syringe (where 100 units = 1mL), 0.4mL converts to 40 units

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Then, lightly pat to enhance absorption

For those using calculators to dial in their protocol, the peptide calculator for weight loss can help with the math

LC-MS of insulin incubated with and without SF in human plasma

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