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For the final steps of mutant construction and confirmation, recombinant colonies were selected by plating on 7H10-ADC with the appropriate antibiotic/supplement and confirmed by PCR using specific primers (Supplementary Table 2 and Fig

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Cryo-EM grid preparation and data acquisition The purified DDB1-DDA1-DET1 complex was prepared at a concentration of 0.2 mg/ml and applied to glow-discharged UltrAuFoil (1.2/1.3, 300 mesh) grid, with an incubation time of 10 sec

Always consult your physician before starting treatment