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To compare changes across the 24 hour period, a one-way ANOVA test was first conducted to determine statistical significance

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For MGMT staining, slides were blocked with 5% goat serum in PBS for 1 h at room temperature (RT), and subsequently incubated with primary antibody (5 g/ml rabbit anti-MGMT, PA5-79668, ThermoFisher Scientific, Germany), for 1 h at RT