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The phase II independent validation cohort (Figure 1B) consisted of 51 men who were receiving chemotherapy (Docetaxel 75 mg m 2 3-weekly, Cabazitaxel 25 mg m 2 3-weekly or Mitoxantrone 12 mg m 2 3-weekly) for mCRPC, who had blood samples obtained prior to commencing chemotherapy and just prior to their second cycle of treatment and were enrolled between April 2009 and September 2012 from hospitals in the PRIMe (Pharmacogenomic Research for Individualized Medicine) network: Sydney Cancer Centre (Royal Prince Alfred Hospital and Concord Hospital), the SAN Clinic, Westmead Hospital and the Mater Misericorde Newcastle Hospital