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On the following day, the dialysed eluate was purified by anion-exchange chromatography on either a Resource Q column (Cytiva, 17-1179-01) or a HiTrap Q XL column (Cytiva, 17515901) pre-equilibrated in buffer A (50 mM Tris pH 8.0, 100 mM NaCl, 5% (v/v) glycerol and 4 mM BME)

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Co-immunoprecipitations To confirm the physical interaction between DDX3X and pU L 31, HeLa cells were grown on 100 mm dishes, mock-treated or infected with HA-tagged U L 31 virus (vJB90) or wild-type strain 17 + at an MOI of 5 for 12 h

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