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10.1164/rccm.201511-2172OC 115

Gene expression analysis showed that a subset of tissue fibrosis-associated genes, (encoding TGF-2 and TGF-3 ( Tgfb2 and Tgfb3 ), SMAD family members 2 and 3 ( Smad2 and Smad3 ), and snail family zinc finger 2 ( Snai2 )) was upregulated markedly in the bladders of IC group rats, and the activation of these TGF- pathway genes except Snai2 was attenuated by NAC injection (Fig