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The recombinant strain ET01 was chosen for further genetic manipulation

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Some of the major benefits were, increased target drug concentrations, low individual variability, decreased gastrointestinal transit time variation, decreased after effects, low chances of dose dumping, various routes of administration (oral, parenteral and inhalation), suitable for hydrophilic and hydrophobic drug loading [316]

For primary mouse hepatocytes isolation, male mice were anticoagulated with heparin sodium and anesthetized with chloral hydrate by intraperitoneal injection