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in Encyclopedia of Metalloproteins (eds Kretsinger, R

10.1161/ATVBAHA.119.312705 242 Van den BosscheJ.O'NeillL

The cells were grown in Luria-Bertani (LB) medium at 37 C and expression of the fusion protein induced by the addition of 0.1 mM isopropyl--D-1-thiogalactopyranoside (IPTG) followed by overnight incubation at 18 C

Drug delivery systems based on HA also face difficulties with controlling release kinetics, ensuring efficient cellular uptake, and overcoming tumor heterogeneity, as not all cancer cells overexpress CD44 receptors targeted by HA conjugates