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The l-EV fraction from FLAG-MIM I-BAR- and Halo7-Rac1-co-expressing cells was serially diluted in the lysis buffer, and the Rac1 amount was measured by ELISA simultaneously with the cell lysates

One tool worth exploring involves metaphor, discussed under the Therapeutic Disclosure section that follows

Entropic stabilization of the tryptophan synthase -subunit from a hyperthermophile, Pyrococcus furiosus : X-ray analysis and calorimetry

and Cao, J

One may also look at using natural forms of folate, rather than synthetic folic acid

Therefore, for each pH both buffers were mixed in a 1:1 ratio and the pH of this solution was measured such that the final pH was known