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Description

The resulting formazan crystals were dissolved in 100 L of dimethyl sulfoxide (DMSO) with gentle shaking for 5 min, and absorbance was subsequently measured at 570 nm using a microplate reader

3A) and the lung injury score (Fig

Chitraju, C

Early signs and symptoms include rash sometimes like a bulls eye rash (Erythema migrans rash), fever, chills, headache, fatigue, muscle and joints aches and swollen lymph nodes

the reduced dye then undergoes non-enzymic oxidation in air, so the addition of a relatively small amount of methylene blue will effectively deplete NADPH, and would be expected to stimulate any pathway that reduces NADP+ to NADPH