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Purification of FLVCR2 for cryo-EM Frozen membranes were thawed on ice and solubilized in basis buffer (200 mM NaCl, 50 mM TrisHCl, pH 7.5, 10% glycerol, 5 mM choline chloride, 20 mM imidazole) containing 0.7% DDM and 0.07% CHS for 1 h at 4C

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