homocysteine reduced glutathione Methylation and Homocysteine metabolism and glutathione (GSH)

Human fibroblasts (purchased from Bonyakhteh Stem Cell Research Center, Tehran, Iran) were cultured according to a similar protocol for the MSC culture

Possible DSIP Side Effects and Safety Concerns Because modern human safety data is limited, the full risk profile of DSIP is not well established

PMID: 17041095

The membranes were incubated with the primary antibodies, namely, Pink1 (1:1000 dilution, Proteintech, United States, 23274-1-AP), LC3 (1:1000 dilution, Cell Signaling Technology, United States, 12741T), sequestosome 1 (SQSTM1/p62) (1:1000 dilution, Cell Signaling Technology, United States, 5114T), Parkin (1:2000 dilution, Proteintech, United States, 14060-1-AP), Kelch-like ECH-associated protein 1 (Keap1) (1:1000 dilution, Proteintech, United States, 60027-1-Ig), nuclear factor E2-related factor 2 (Nrf2) (1:2000 dilution, Proteintech, United States, 16396-1-AP), B- cell lymphoma - 2 (Bcl-2) (1:2000 dilution, Proteintech, United States, 26593-1-AP), Bcl - 2-associated X protein (Bax) (1:5000 dilution, Proteintech, United States, 50599-2-Ig), Cytochrome C (Cyto C) (1:5000, Proteintech, United States, 10993-1-AP), Caspase 3 (1:1000 dilution, Proteintech, United States, 19677-1-AP) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:500000 dilution, Proteintech, United States, 60004-1-Ig)

Eur J Clin Nutr 2000;54:424-8

Jensen, RT, Wang, LH , Lin, JT, Benya, RV, Mroziski, JE, Jr, Coy, DH