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Statistics analysis Statistical analysis was performed using GraphPad Prism 8.0 (GraphPad Software) and presented as means SEM

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At timepoint 360 min, we observed a pronounced drop in both fully labeled glucose and lactate in iPSCs, most likely reflecting depletion of the extracellular glucose supply (5 mM) in the medium, which may have contributed to the onset of gluconeogenesis, highlighting a metabolic adaptation in response to nutrient availability [67]

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Evening administration before bed aligns with natural growth hormone rhythms and appears to produce optimal results