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For passaging of organoids, the collagen domes were incubated with 1 mg ml 1 collagenase from Clostridium histolyticum (Sigma-Aldrich) for 30 min at 37 C, and digestion monitored using bright-field microscopy (small clumps of cells)

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When the concentration of glutathione in milk and ewe's milk was 0.02 mg/mL, the IR characteristic absorption peaks at 1,654 and 1,533 cm 1 were still visible, which were generated by the carbonyl C=O bond stretching (amide I) and CNH bond bending (secondary amide II) vibrations, respectively