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CS and GAPDH were used as loading controls

It is designed to bind GHRH receptors on pituitary somatotroph cells, prompting the body's own pituitary gland to release growth hormone (GH), which in turn raises downstream IGF-1 levels

EOPBM employed a pulsed diode laser (Gemini Diode Laser, Azena Medical LLC, USA) with dual wavelengths (810/980 nm), targeting 10 points across the face and anterior neck, including bilateral submandibular and submental regions (Table 3)

All calculations were carried out with the B3LYP functional 50,51 , augmented with Grimmes D3 dispersion correction 52,53 , which already proved to be a good choice for chalcogen-containing systems 54,55

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