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Non-specific elution is performed by changing solvent conditions like pH, ionic strength and polarity

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Unless indicated separately, the cells were seeded in the RPMI-1640 medium (Corning) supplemented with 10% FBS, or heat-inactivated dialysed FBS (DFBS), 2 mM l-glutamine, 1% sodium pyruvate (Sigma-Aldrich), 100 units/ml -1 penicillin, 100 g/ml -1 streptomycin and 0.05 mM 2-mercaptoethanol (Sigma-Aldrich) at 37 C and 5% CO 2

Mechanisms of antimicrobial resistance in ESKAPE pathogens