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see panel d inset for the culture design)

Gel electrophoresis and immunoblotting Whole-cell lysate samples were prepared by lysing cells in RIPA buffer with phosphatase and protease inhibitors (Pierce protease and phosphatase inhibitor tablets) after washing three times with dPBS

Lin D, Tucker MJ, Rieder MJ

doi: 10.3390/antiox12091732 164 ReisJGorgullaCMassariMMarcheseSValenteSNoceBet al

Figure 5 4 Discussion The widespread application of semen cryopreservation technology has effectively prolonged sperm lifespan, overcoming temporal and geographical constraints while maximizing the preservation of superior genetic resources from elite breeding males