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The absorbance was measured at 450 nm using a 96-well plate reader (Dynex Tech., Chantilly VA., USA)

10.1001/jamapsychiatry.2014.62 59

The ActA protein may have other functions, e.g., binding heparan sulfate on the cell surface, which allows attachmemt and entering host cells cultured in vitro (Surez et al., 2001)