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Based on the four dimensions of oral feasibility, gastric retention efficacy, difficulty in large-scale production, and regulatory acceptability, we can divide the existing nanomaterials into three progressive levels: Tier 1: pH-responsive monofunctional nanomaterials

batch 10 (figure 7C) and batch 11 (figure 7D)

doi: 10.3389/frph.2022.822257 Received 25 November 2021 Accepted 17 January 2022 Published 22 February 2022 Volume 4 - 2022 Edited by Roland Eghoghosoa Akhigbe, Ladoke Akintola University of Technology, Nigeria Reviewed by Giulia Collodel, University of Siena, Italy

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To decipher the exact subcellular localization of AuNPs-d-PLL-PEG-FA.siRNA 50 nM, cells were incubated with the NP complexes for 4 hours at 37 C