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Cells were then centrifuged, and the resulting cell pellets were resuspended in DMEM (without phenol red and serum) containing DAF-FM-DA (10 M, for NO detection), DCFH-DA (10 M, for ROS detection) or BODIPY-581/591-C11 (10 M, for lipid-ROS detection)

In vivo and in vitro studies suggest that ferroptosis may contribute to DM-related cognitive dysfunction, causing neuronal damage in diabetic animals

The titrations were conducted using 17 successive injections of 2.4 L (the first at 0.4 L and the remaining 16 at 2.4 L) with a duration of 4 s per injection and a spacing of 180 s between injections

The NOAEL for these effects was 125 mg -tocopheryl acetate/kg body weight (Abdo et al., 1986)

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