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RTPCR and semiquantitative RTPCR To determine VEGFA isoform and receptor expression, RNA was isolated from the lens and RPE/choroid with TRIzol reagent (Life Technologies)

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doi: 10.3389/fphar.2021.665031 Received 06 February 2021 Accepted 07 June 2021 Published 17 June 2021 Volume 12 - 2021 Edited by Philippe De Deurwaerdere, Universit de Bordeaux, France Reviewed by Karl Tsim, Hong Kong University of Science and Technology, China Touqeer Ahmed, National University of Sciences & Technology, Pakistan Abhijit Dey, Presidency University, India Updates Copyright 2021 Islam, Quispe, Hossain, Islam, Al-Harrasi, Al-Rawahi, Martorell, Mamurova, Seilkhan, Altybaeva, Abdullayeva, Docea, Calina and Sharifi-Rad

Mitochondrial lysate (~30 g protein) was diluted in 200 l of assay buffer (1 mM MgCl 2 , 400 M thiamine diphosphate, 50 mM HEPES, 10% glycerol, 0.05% BSA) and combined with substrates (-KG, CoA, NAD + )

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Organic acids inhibit the growth of pathogenic bacteria by increasing their outer membrane permeability, altering intracellular osmotic pressure, and inhibiting DNA synthesis