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These disruptions can lead to adverse effects such as diarrhea and damage to the intestinal lining

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Then, the bound oligonucleotides were eluted by incubating the binding complex three times with 200 L elution buffer (40 mM Tris-HCl pH 8, 10 mM EDTA, 3.5 M urea, 0.02% Tween 20) at 90 C for 10 minutes