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In contrast, the ZVAD co-treatment failed to suppress the uptake of PI in RSL3-treated 3D PTC spheroids, mirroring tumor cell viability findings in 2D

name SD medium medium (unbuffered) pH 6.0 BY4743 Parent 4.6 0.4 0.5 0.2 yjll53c inol 40 3 27 3 y oil 08c ino4 15 3 22 2 ypl002c vps22 50 1 22 4 ygll67c pmrl 30 4 15 4 ylrll48w pep3 50 1 14 2 ylr226w bur2 14 3 12 2 ylrl c efr4 44 4 12 3 ybr279w pafl 25 1 9 2 ybr289w sn 19 1 8 1 ylr322w 20 2 6 1 ylr396c vps33 24 5 5 0.3 yhl025w snf 13 7 5 0.1 ygll27c sohl 10 7 4 1 ydr323c pep7 31 7 4 1 ydr495c vps3 23 7 4 1 ylr025w vps32 40 7 4 1 ycr063w bud31 11 1 4 1 ynl215w 7 1 3.4 0.3 ymr077c vps20 35 5 3.5 0.5 yor036w pepl2 35 2 3.0 0.4 ylr261c 18 3 3 1 These results also suggest that the combination of mutations where glutathione production is highly dependent on external pH, with those that are less-dependent on external pH could produce cells that produce even higher levels of glutathione

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c , Targeting scheme for Slc25a39 conditional knockout (Slc25a39 fl/fl ) mice in which two loxp sites were inserted in the indicated intronic regions

Moreira et al., 2022

4-Hydroxynonenal metabolites and adducts in pre-carcinogenic conditions and cancer