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However, the most common presentation of CP-induced nephrotoxicity is acute kidney injury (AKI) (Miller et al., 2010), which is characterized by the rapid decline of renal excretory function leading to the accumulation of nitrogenous waste products, water, and electrolytes (Ostermann and Joannidis, 2016

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PhoA accumulation was analyzed by SDS-PAGE (NuPAGE 4 bis 12 %, Bis-Tris, Invitrogen, Waltham, MA, USA) and Western blot analyses were carried out using nitrocellulose membranes in the iBLOT2 system (Invitrogen, Waltham, MA, USA), both according to the supplier

doi: 10.1016/S1474-4422(14)70144-9 33

These combined effects help improve glycaemic control and often result in significant weight loss, though weight management is not a licensed indication in the UK