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Tandem mass tags: A novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS

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Abstract Within the consistent daily rhythm of human life, intervertebral discs endure a variety of complex loads beyond the influences of gravity and muscle forces, leading to significant morphological changes (in terms of volume, area, and height) as well as biomechanical alterations, including an increase in disc stiffness and a decrease in intradiscal pressure