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Following incubation with the primary antibody recognizing the antigen of interest and subsequent PBS washing, a biotinylated secondary antibody was introduced, which was then complexed with streptavidin-horseradish peroxidase (HRP)

25% for sorafenib and placebo, respectively

WCV treatment significantly reduced the percentage of M1 macrophages (29.13% in the WCV group) and increased the percentage of M2 macrophages (26.47% in the VM group), which was close to the control in the VM group, whereas anti-PD-1 antibody treatment had no significant rescue effect in the PM group (M1, 33.85% and M2, 25.93%)

Exposure of Brassica juncea (L) to arsenic species in hydroponic medium: comparative analysis in accumulation and biochemical and transcriptional alterations